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Agilent 2100 Bioanalyzer (english version)

About the training

We have prepared this privatized online course (SPOC), organized into several modules, so that you become self-sufficient in the use of the Bio-analyzer. At each step, you will be invited fill-in a memory quiz, and, at the end of the training, you will get your certificate, allowing you to have access to the Bio-analyzer of the platform. This training is accessible 24 hours a day and the training time lasts about 2 hours.

Thematics

Bio-analyzer Agilent, nucleic acids analysis, quality control, RNA, DNA...

Course plan

Module 1 System overview
        Module 1 Part 1: principles
        Module 1 Part 2: advantages
        Module 1 part 3: choosing the right chip
Module 2 Preparation of a Nano chipbr>         Module 2 Part 1: realization of a Nano chip
        Module 2 Part 2: The software - Launching a run and obtaining results
Module 3 Troubleshootings
        Module 3 Part 1: Troubleshootings with the chip
        Module 3 Part 2: Troubleshootings with the run
Module 4: Administrative and practical aspects

Documentation

The protocols are downloadable from the " protocols " tab. Protocols. The transcription of the videos is available at the bottom of each module.

Trainers

The trainers belong to the Biomics pole, integrated to CITECH and are in charge of the projects using the Next Generation Sequencing technology (NGS). The pole manages many projects of service and collaboration.

Course Staff Image #1

Caroline Proux

Caroline Proux is holding a DESS in "Ressources génomiques et traitements informatiques". She firstly worked at INRA and joined the platform in 2009 where, as an engineer, she was involved in transcriptomics projects based on DNA microarray technology. Since 2010, she has been in charge of many projects aiming at gene expression profiling by Next Generation Sequencing (RNAseq, ChIPseq) from the quality control of the samples to the generation of the sequencing data. She is also deeply involved in user training and technological survey activities.

To contact her, please send an e-mail at the address "karo@pasteur.fr".

Course Staff Image #2

Odile Sismeiro

Odile Sismeiro is holding a "Diplôme Universitaire de Technologie" and a "Diplôme d'Etudes Scientifiques et Techniques" (Conservatoire des Arts et Métiers, CNAM, Paris). She joined Institut Pasteur in 1987 and has been working for 13 years on the regulation of genetic expression in bacteria at Antoine Danchin's research unit. Then she joined the "Microarrays" platform. She firstly contributed to the development of the DNA array technology at the platform and, in 2009, she established Next Generation Sequencing. Since then, as an engineer, she has been leading many projects aiming at gene expression profiling, from the quality control of the nucleic acids to the generation of sequencing data. She also has important user training and technological development activities. She is taking a very active part in communication.

To contact her, please send an e-mail at the address "osisme@pasteur.fr".

Concerned audience

This MOOC is meant for all the persons having an IP e-mail address (…..@pasteur.fr) and who wishes to use the Agilent Bio-analyzer in full autonomy at the platform.

  1. Course Number

    CM03
  2. Classes Start

    Jun 29, 2018
  3. Classes End

    Sep 05, 2030
  4. Estimated Effort

    2:00
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