About the training
We have prepared this privatized online course (SPOC), organized into several modules, so that you become self-sufficient in the use of the Bio-analyzer. At each step, you will be invited fill-in a memory quiz, and, at the end of the training, you will get your certificate, allowing you to have access to the Bio-analyzer of the platform. This training is accessible 24 hours a day and the training time lasts about 2 hours.
Bio-analyzer Agilent, nucleic acids analysis, quality control, RNA, DNA...
Module 1 System overview
Module 1 Part 1: principles
Module 1 Part 2: advantages
Module 1 part 3: choosing the right chip
Module 2 Preparation of a Nano chipbr>
Module 2 Part 1: realization of a Nano chip
Module 2 Part 2: The software - Launching a run and obtaining results
Module 3 Troubleshootings
Module 3 Part 1: Troubleshootings with the chip
Module 3 Part 2: Troubleshootings with the run
Module 4: Administrative and practical aspects
The protocols are downloadable from the " protocols " tab. Protocols.
The transcription of the videos is available at the bottom of each module.
The trainers belong to the Biomics pole, integrated to the C2RT and are in charge of the projects using the Next Generation Sequencing technology (NGS). The pole manages many projects of service and collaboration.
Caroline Proux is holding a DESS in "Ressources génomiques et traitements informatiques". She firstly worked at INRA and joined the platform in 2009 where, as an engineer, she was involved in transcriptomics projects based on DNA microarray technology. Since 2010, she has been in charge of many projects aiming at gene expression profiling by Next Generation Sequencing (RNAseq, ChIPseq) from the quality control of the samples to the generation of the sequencing data. She is also deeply involved in user training and technological survey activities.
Caroline left the platform in october, 2018.
Odile Sismeiro is holding a "Diplôme Universitaire de Technologie" and a "Diplôme d'Etudes Scientifiques et Techniques" (Conservatoire des Arts et Métiers, CNAM, Paris). She joined Institut Pasteur in 1987 and has been working for 13 years on the regulation of genetic expression in bacteria at Antoine Danchin's research unit. Then she joined the "Microarrays" platform. She firstly contributed to the development of the DNA array technology at the platform and, in 2009, she established Next Generation Sequencing. Since then, as an engineer, she has been leading many projects aiming at gene expression profiling, from the quality control of the nucleic acids to the generation of sequencing data. She also has important user training and technological development activities. She is taking a very active part in communication.
To contact her, please send an e-mail at the address "firstname.lastname@example.org".
This MOOC is meant for all the persons having an IP e-mail address (…..@pasteur.fr) and who wishes to use the Agilent Bio-analyzer in full autonomy at the platform.